Purified anti-GFP

The Green Fluorescent Protein (GFP) was originally identified as a protein involved in the bioluminescence, which is from the jellyfish Aequorea Victoria. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces without the need for exogenous substrates or cofactors, or other intrinsic or extrinsic proteins, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Purified GFP is a 27 kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

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SPECIFICATIONS

Catalog Number

338001

Size

25 microg

Applications

FC, ICC, IP, WB

Hosts

Rat

Reactivities

Hum

Clone

FM264G

Antigen

TLR9GFP expressed cell line

Isotype

Rat IgG2a, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ? 0.25 microg per 106 cells in 100 microl volume or 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

Green Fluorescent Protein

Regulatory Status

RUO