Purified anti-GAPDH

This mouse monoclonal GAPDH antibody recognizes human GAPDH, also known as glyceraldehyde-3-phosphate dehydrogenase. GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases. The FF26A/F9 monoclonal antibody has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

649202

Size

100 microg

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Clone

FF26A/F9

Antigen

Human CD4 lymphocytes

Gene Id

5590

Isotype

Mouse IgG1

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.1-0.5 microg antibody per 1 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store undiluted at 4C.

Alternative Names

glyceraldehyde-3-phosphate dehydrogenase

Regulatory Status

RUO