Purified anti-Fyn

Fyn is a 59 kD protein tyrosine kinase and a member of the src subfamily. There are two reported isoforms of Fyn; FynT found in T cells, B cells and thymocytes, and FynB found in neuronal cells. Fyn has been shown to control cell growth, interact with the p85 subunit of PI3K, control high affinity IgE receptor-mediated mast cell degranulation, and participate in neuronal cell signaling. Fyn can be activated by T cell receptor engagement or anti-CD3 ligation. Fyn is known to be modified by phosphorylation and palmitoylation and has been shown to interact with Fyn binding protein, p85 PI3K, high affinity IgE receptor, and NCAM140. The FYN-59 monoclonal antibody recognizes human and mouse Fyn and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.

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SPECIFICATIONS

Catalog Number

626501

Size

25 microg

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Clone

FYN-59

Antigen

Partial recombinant human Fyn (aa 7-176) expressed in E coli

Gene Id

2534

Isotype

Mouse IgG

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 microg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store antibody solution undiluted at 4C.

Alternative Names

Tyrosine kinase p59fyn, Protein-tyrosine kinase fyn, Proto-oncogene tyrosine-protein kinase fyn

Regulatory Status

RUO