Purified anti-FOXP3

The forkhead/winged helix transcription factor FOXP3 is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. The Poly6238 antibody recognizes both human and mouse FOXP3 and has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

623802

Size

200 microl

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Clone

Poly6238

Antigen

Peptide-KLH

Gene Id

50943

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl antibody per 5 ml antibody dilution buffer for each mini-gel. If primary CD4+CD25+ cells are used, it is recommended that 0.5-1 x 106 cell equivalents be loaded per lane. For immunohistochemical staining of paraffin-embedded sections, the suggested use of this reagent is 1:20 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store undiluted at -20 C.

Alternative Names

Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2

Regulatory Status

RUO