Purified anti-eIF4E

eIF4E (also known as eukaryotic translation initiation factor 4E) is a 25 kD member of the eukaryotic initiation factor 4E family. This ubiquitously expressed cytoplasmic protein recognizes and binds 7-methylguanosine containing mRNA "CAP" during initiation of protein synthesis. eIF4E facilitates ribosome binding by inducing unwinding of mRNA secondary structures. Activity of this protein is inhibited by binding of 4E-BP and PRH; expression is increased by H2O2 treatment. Activity can also be regulated by integrin alpha6beta4 ligation. Phosphorylation increases binding to mRNA CAPs and formation of eIF4F complex. eIF4E forms a trimeric complex with eIF4G, eIF4A (called eIF4F). Also associates with 4E-BP and PRH. The Poly6069 antibody has recognizes human eIF4E and has been shown to be useful for Western blotting.