Purified anti-Cytokeratin 18

Keratin 18 (also know as cytokeratin 18) is a 48 kD member of the Type I intermediate filament family, that forms heterotetramers. Keratin 18 is widely expressed in normal single layer epithelial tissues (liver, pancreas) and is expressed at high levels in some tumor tissues. Keratin 18 and filament partner keratin 8 are the most common members of intermediate filament family. Mutations in keratin 18 have been linked to cryptogenic cirrhosis. This protein is induced by retinoic acid, estrogen (breast cancer cells), and the Ras-signaling pathway (Ha-Ras, activated Src, Lck, Raf). Keratin 18 can be modified by phosphorylation, glycosylation, and ubiquitination. The active heterotetramer consists of two type I and two type II keratins (type I is acidic 40-55 kD, type II is neutral to basic 56-70 kD). Keratin 18 has been shown to associate with keratin 8, Mrj (a DnaJ/HSP40 family protein), and Hsp70. The Poly6172 antibody recognizes human and mouse keratin 18 and is useful for Western blotting.

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SPECIFICATIONS

Catalog Number

617201

Size

50 microl

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Clone

Poly6172

Antigen

Recombinant (partial), C-terminal

Gene Id

3875

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store frozen at -20 C.

Alternative Names

Cytokeratin 18, Keratin type I cytoskeletal 18

Regulatory Status

RUO