Purified anti-Cytochrome b-245 heavy chain

The integral membrane protein flavocytochrome b (Cyt b), a heterodimer formed by a 91-kD glycoprotein (heavy chain) and a 22-kD polypeptide (light chain), is the catalytic core of the phagocyte NADPH oxidase and generates superoxide which plays a critical role in host defense. NADPH oxidase is a central well characterized component of the innate immune response against fungal and bacterial infections. Recent research also offers new insight into a novel role for this oxidase in the coordination of the adaptive immune response. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.

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SPECIFICATIONS

Catalog Number

650101

Size

25 microg

Applications

ELISA, FC, IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Clone

54.1

Antigen

Partially purified human neutrophil flavocytochrome b

Gene Id

4519

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody has been quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 microg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store undiluted at 4C.

Alternative Names

CYBB, gp91phox, cytochrome b-245 beta chain, Superoxide-generating NADPH oxidase heavy chain subunit

Regulatory Status

RUO