Purified anti-Cyclin E

This rabbit polyclonal antibody recognizes human, mouse, and rat cyclin E also known as Cyclin E1, CCNE, G1/S specific cyclin E1. Cycline E is a nucleus protein, predicted molecular weight 47 kD. Cycline E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cycline E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E is essential for the control of the cell cycle at the G1/S transition. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. The poly6307 antibody has been shown to be useful for Western blotting, Immunoprecipitation, and Immunohistochemistry.

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SPECIFICATIONS

Catalog Number

630701

Size

125 microl

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Clone

Poly6307

Antigen

Peptide-KLH (GVLTPPHSSKKQSSEQETE)

Gene Id

25729

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 1:100~400 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:100 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

Cyclin E1, CCNE, G1/S specific cyclin E1

Regulatory Status

RUO