Purified anti-Bax

Bax is a 21 kD pro-apoptotic protien known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, and nucleus and is highly expressed in hematopoietic stem cells, the ovary, and in the lymph node. Bax binds the anti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins such as Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptotic stimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome c to pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionally activate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers. The Poly6251 antibody has been reported to be useful for Western blotting, immunoprecipitation, and immunohistochemistry of human, mouse, and rat Bax protein.

Prices direct from BioLegend

Quick response times

Exclusive Absave savings/discounts

SPECIFICATIONS

Catalog Number

625101

Size

50 microl

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Clone

Poly6251

Antigen

Peptide-KLH, N-terminal

Gene Id

581

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C. DO NOT FREEZE.

Alternative Names

BCL2 associated X protein, apoptosis regulator Bax

Regulatory Status

RUO