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Products Polyclonal Anti-UCP1
| Catalogue number: | PA1981 |
| Price: | $200.00 |
| Reactivities: | Human |
| Applications: | Western Blot |
| Size: | 100ug/vial |
| Gene: | UCP1 |
| Swiss prot: | P25874 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing. |
| Scientific background: | UCP1 (Uncoupling Protein 1), also called THERMOGENIN or UCP, is an uncoupling protein found in the mitochondria of brown adipose tissue (BAT). Using in situ hybridization, Cassard et al. (1990) assigned the human UCP gene to 4q31. They found that the primary structure of UCP is similar to that of ADP/ATP translocator of skeletal muscle, the gene for which is also located on chromosome 4. The failure of UCP1 expressed in E. coli inclusion bodies to carry out fatty acid-dependent H+ transport activity inclusion bodies made Echtay et al. (2000) seek a native UCP cofactor. They identified coenzyme Q (CoQ, or ubiquinone) as such a cofactor. On addition of CoQ(10) to reconstituted UCP1 from inclusion bodies, fatty acid-dependent proton transport reached the same rate as with native UCP1. |
| References: | 1. Cassard, A. M., Bouillaud, F., Mattei, M. G., Hentz, E., Raimbault, S., Thomas, M., Ricquier, D. Human uncoupling protein gene: structure, comparison with rat gene, and assignment to the long arm of chromosome 4. J. Cell. Biochem. 43: 255-264, 1990. 2. Echtay, K. S., Winkler, E., Klingenberg, M. Coenzyme Q is an obligatory cofactor for uncoupling protein function. Nature 408: 609-613, 2000. |
| Additional info: | A synthetic peptide corresponding to a sequence at the C-terminal of human UCP1 |
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