Polyclonal Anti-TSHR

Price

200.00 USD

Size

100ug/vial

Reactivities

Hum

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Akamizu, T., Ikuyama, S., Saji, M., Kosugi, S., Kozak, C., McBride, O. W., Kohn, L. D. Cloning, chromosomal assignment, and regulation of the rat thyrotropin receptor: expression of the gene is regulated by thyrotropin, agents that increase cAMP levels, and thyroid autoantibodies. Proc. Nat. Acad. Sci. 87: 5677-5681, 1990. \n2. Dechairo, B. M., Zabaneh, D., Collins, J., Brand, O., Dawson, G. J., Green, A. P., Mackay, I., Franklyn, J. A., Connell, J. M., Wass, J. A. H., Wiersinga, W. M., Hegedus, L., Brix, T., Robinson, B. G., Hunt, P. J., Weetman, A. P., Carey, A. H., Gough, S. C. Association of the TSHR gene with Graves' disease: the first disease-specific locus. Europ. J. Hum. Genet. 13: 1223-1230, 2005.\n3. Wadsworth, H. L., Chazenbalk, G. D., Nagayama, Y., Russo, D., Rapoport, B. An insertion in the human thyrotropin receptor critical for high affinity hormone binding. Science 249: 1423-1425, 1990.\n

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Scientific Background

TSHR (Thyroid-Stimulating Hormone Receptor), also called LGR3. Akamizu et al. (1990) mapped the mouse Tshr gene to chromosome 12 using linkage studies in interspecies backcross mice. The TSH receptor differs from the LHCG receptor by the presence of 2 unique insertions of 8 and 50 amino acids in the extracellular domain. Wadsworth et al. (1990) showed that the 8-amino acid tract near the amino terminus of the TSH receptor is an important site of interaction with both TSH and autoantibodies against the TSH receptor (thyroid-stimulating immunoglobulins, TSI).