Polyclonal Anti-HTR3A

Price

200.00 USD

Size

100ug/vial

Reactivities

Hum

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1.Bruss, M., Eucker, T., Gothert, M., Bonisch, H. Exon-intron organization of the human 5-HT-3A receptor gene. Neuropharmacology 39: 308-315, 2000. \n2.Kelley, S. P., Dunlop, J. I., Kirkness, E. F., Lambert, J. J., Peters, J. A. A cytoplasmic region determines single-channel conductance in 5-HT(3) receptors. Nature 424: 321-324, 2003.\n3.Maricq, A. V., Peterson, A. S., Brake, A. J., Myers, R. M., Julius, D. Primary structure and functional expression of the 5HT-3 receptor, a serotonin-gated ion channel. Science 254: 432-437, 1991.\n

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Scientific Background

HTR3A (5-HYDROXYTRYPTAMINE RECEPTOR 3A), also called SEROTONIN 5-HT-3 RECEPTOR, is a member of the ligand-gated ion channel receptor superfamily. HTR3A is a neurotransmitter in the central and peripheral nervous systems that plays a role in many physiologic processes such as sleep, appetite, thermoregulation, pain perception, hormone secretion, and sexual behavior. Using fluorescence in situ hybridization, the HTR3 gene is assigned to 11q23.1-q23.2. The single-channel conductance for homomers of HTR3A is 0.4 pS. Replacement of 3 arginine residues unique to the HA-stretch of the HTR3A subunit by their HTR3B subunit counterparts increases single-channel conductance 28-fold. Ultrastructural studies of the Torpedo nicotinic acetylcholine receptor indicated that the key residues might frame narrow openings that contribute to the permeation pathway. The genomic structure of the HTR3A gene contains 7 exons and spans approximately 14.5 kb.