PerCP/Cy5.5 anti-human CD45

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

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SPECIFICATIONS

Catalog Number

304027

Size

25 tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Clone

HI30

Gene Id

5788

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this CD45 antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 microl per million cells or 5 microl per 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximum emission of 690 nm.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The CD45 antibody solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.

Alternative Names

LCA, T200

Regulatory Status

RUO