PE anti-mouse/rat/human FOXP3 Flow Kit

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 delta2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2. BioLegend's PE anti-mouse/rat/human FOXP3 Flow Kit is designed and formulated specifically for immunofluroscence staining and flow cytometric analysis of mouse/rat/human FOXP3+ Treg cells in a mixed lymphocyte population. This kit is composed of PE conjugated anti-mouse/rat/human FOXP3 antibody and PE conjugated matching isotype control and the critical buffers. It is easy to use for identification of FOXP3+ Treg cells.