PE anti-mouse Ikaros

Ikaros family members, including Ikaros and Helios, play a role in the development of lymphocytes, B- and T-cells. They are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T-cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T-cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and IL-2 mediated signaling events.

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SPECIFICATIONS

Catalog Number

653304

Size

100 microg

Applications

FC, ICC

Hosts

Mouse

Reactivities

Mouse

Clone

2A9/Ikaros

Antigen

N-terminal half of mouse Ikaros fused to GST.

Gene Id

22778

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?0.015 microg per million cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.2 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Alternative Names

IKAROS family zinc finger 1, DNA-binding protein Ikaros

Regulatory Status

RUO