PE anti-human FOXP3

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 delta2) lacking exon 2. The 206D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.

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SPECIFICATIONS

Catalog Number

320108

Size

100 tests

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

Clone

206D

Antigen

full-length FOXP3 protein

Gene Id

50943

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. Test size products are transitioning from 20 microl to 5 microl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microl staining volume or per 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The FOXP3 antibody solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.

Alternative Names

Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2

Regulatory Status

RUO