Pacific Blue(TM) anti-mouse CD8a

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked alpha and beta chains that form the alpha(CD8a)/beta(CD8b) heterodimer and alpha/alpha homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 alpha/beta heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR alpha/beta T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 alpha/alpha homodimer is expressed on a subset of gamma/delta TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

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SPECIFICATIONS

Catalog Number

100728

Size

25 microg

Applications

FC

Hosts

Rat

Reactivities

Mouse

Clone

53-6.7

Antigen

Mouse thymus or spleen

Gene Id

12525

Isotype

Rat IgG2a, kappa

Clonality

Monoclonal

Dilutions

This CD8a reagent is developed for immunofluorescent staining for flow cytometric analysis; the suggested use of this reagent is ? 0.25 microg per 106 cells in 100 microl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue(TM) has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue(TM) conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue(TM) is a registered trademark of Molecular Probes, Inc. Pacific Blue(TM) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The CD8a antibody solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.

Alternative Names

T8, Ly-2 (Ly2) Lyt-2 (Lyt2)

Regulatory Status

RUO