Pacific Blue(TM) anti-mouse CD4

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

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SPECIFICATIONS

Catalog Number

100534

Size

25 microg

Applications

FC

Hosts

Rat

Reactivities

Mouse

Clone

RM4-5

Antigen

BALB/c mouse thymocytes

Gene Id

12504

Isotype

Rat IgG2a, kappa

Clonality

Monoclonal

Dilutions

This CD4 reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ?1.0 microg per 106 cells in 100 microl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue(TM) has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue(TM) conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue(TM) is a registered trademark of Molecular Probes, Inc. Pacific Blue(TM) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The CD4 antibody solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.

Alternative Names

L3T4, T4

Regulatory Status

RUO