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Pacific Blue(TM) anti-mouse CD3

Pacific Blue(TM) anti-mouse CD3

Cat no: 100213


Supplier: BioLegend
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CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3epsilon, delta, gamma and zeta chains. It forms a TCR complex by associating with TCR alpha/beta or gamma/delta chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Catalogue number: 100213
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry
Size: 25 microg
Clone: 17A2
Gene: 12502
Dilutions: Each lot of this CD3 antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?1.0 microg per 106 cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue(TM) has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue(TM) conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue(TM) is a registered trademark of Molecular Probes, Inc. Pacific Blue(TM) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage buffer: The CD3 antibody solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.5 mg/ml
Antigen: gammadeltaTCR-positive T-T hybridoma D1
Isotype: Rat IgG2b, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: T cell antigen receptor complex, T3