Pacific Blue(TM) anti-mouse CD3

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3epsilon, delta, gamma and zeta chains. It forms a TCR complex by associating with TCR alpha/beta or gamma/delta chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

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SPECIFICATIONS

Catalog Number

100213

Size

25 microg

Applications

FC

Hosts

Rat

Reactivities

Mouse

Clone

17A2

Antigen

gammadeltaTCR-positive T-T hybridoma D1

Gene Id

12502

Isotype

Rat IgG2b, kappa

Clonality

Monoclonal

Dilutions

Each lot of this CD3 antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?1.0 microg per 106 cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue(TM) has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue(TM) conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue(TM) is a registered trademark of Molecular Probes, Inc. Pacific Blue(TM) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The CD3 antibody solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.

Alternative Names

T cell antigen receptor complex, T3

Regulatory Status

RUO