Pacific Blue(TM) anti-human CD45RA

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

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SPECIFICATIONS

Catalog Number

304123

Size

100 tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Clone

HI100

Gene Id

5788

Isotype

Mouse IgG2b, kappa

Clonality

Monoclonal

Dilutions

Each lot of this CD45RA antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.For test size, the suggested use of this reagent for immunofluorescent staining is 20 microl per millioncells in 100 microl volume.For microg sizes,the suggested use of this reagent for immunofluorescent stainingis ?0.5 microg per millioncells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue(TM) has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue(TM) conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue(TM) is a registered trademark of Molecular Probes, Inc. Pacific Blue(TM) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The CD45RA antibody solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.

Regulatory Status

RUO