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Pacific Blue(TM) anti-human CD25

Pacific Blue(TM) anti-human CD25

Cat no: 302620

Supplier: BioLegend
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CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor alpha chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor beta (CD122) and common gamma chains (CD132) to form the high affinity IL-2R complex.
Catalogue number: 302620
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100 microg
Clone: BC96
Gene: 3559
Dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For test size, the suggested use of this reagent for immunofluorescent staining is 5 microl per 106 cells in 100 microl volume.For microg sizes, the suggested use of this reagent for immunofluorescent stainingis ?1.0 microg per 106 cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue(TM) has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue(TM) conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue(TM) is a registered trademark of Molecular Probes, Inc. Pacific Blue(TM) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage buffer: The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.5 mg/ml
Isotype: Mouse IgG1, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Low affinity IL-2R, IL-2R alpha chain, Tac, p55
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