Pacific Blue(TM) anti-human CD16

CD16 is known as low affinity IgG receptor III (FcgammaRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcgammaRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcgammaRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

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SPECIFICATIONS

Catalog Number

302032

Size

100 tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Clone

3G8

Gene Id

2214

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For test size, the suggested use of this reagent for immunofluorescent stainingis 5 microl per 106 cells in 100 microl volume. For microg sizes, the suggested use of this reagent for immunofluorescent stainingis ?1.0 microg per 106 cells in 100 microl volume.It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue(TM) has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue(TM) conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue(TM) is a registered trademark of Molecular Probes, Inc. Pacific Blue(TM) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Alternative Names

FcgammaRIII

Regulatory Status

RUO