Pacific Blue(TM) anti-human CD14

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

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SPECIFICATIONS

Catalog Number

301815

Size

100 microg

Applications

FC

Hosts

Mouse

Reactivities

Hum

Clone

M5E2

Gene Id

929

Isotype

Mouse IgG2a, kappa

Clonality

Monoclonal

Dilutions

This reagent is developed for immunofluorescent staining for flow cytometric analysis. For test size, the suggested use of this reagent for immunofluorescent staining is 5 microl per million cells or 5 microl per 100 microl of whole blood. For microg sizes,the suggested use of this reagent is ? 2.0 microg per 106 cells in 100 microl volume or 100 microl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue(TM) has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue(TM) conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue(TM) is a registered trademark of Molecular Probes, Inc. Pacific Blue(TM) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Alternative Names

LPS receptor

Regulatory Status

RUO