This antibody is reactive with Notch 1 by western blotting. A strong response was also detected by ELISA against the immunizing peptide.This product was assayed by immunoblot against recombinant mouse Notch constructs in 293 cells. An 80 kDa band corresponding to Notch 1 was observed at a 1:500 dilution. This product was also assayed against the peptide immunogen in a standard capture ELISA using Peroxidase conjugated anti-Rabbit IgG (H&L) [Goat] (code #611-1302) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (code # ABTS-100) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:60,000 is suggested for this product.
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