Neuraminidase Antibody

This antibody is suitable for western blotting, immunocytochemistry, immunoprecipitation, transfected cell culture, primary cell culture, and ELISA. This product was assayed on immunoblots against human primary fibroblast cell lysates. A single band of the expected apparent molecular weight was observed at a 1:500 dilution incubated for 2 h at room temperature. For immunofluorescence microscopy a 1:500 dilution is recommended incubated for 2 h at room temperature or alternatively, a 1:1,000-1:5,000 dilution can be used incubated overnight at room temperature. Staining is best on paraformaldehyde-fixed cultures. The optimal dilution and time of incubation is dependent on the methods used, the type of cells or tissue, and the amount of protein present. Neuraminidase is not very abundant in most tissues and its detection with the antibodies may require further optimization. Researchers should determine optimal titers for other applications.