The Micro BCA Protein Assay Kit is a detergent-compatible bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein. An adaptation of the BCA Protein Assay Kit, the Micro BCA Kit has been optimized for use with dilute protein samples (0.5-20 ug/ml). The unique, patented method utilizes bicinchoninic acid (BCA) as the detection reagent for Cu+1, which is formed when Cu+2 is reduced by protein in an alkaline environment. A purple-colored reaction product is formed by the chelation of two molecules of BCA with one cuprous ion (Cu+1). This water-soluble complex exhibits a strong absorbance at 562 nm that is linear with increasing protein concentrations. The macromolecular structure of protein, the number of peptide bonds and the presence of four amino acids (cysteine, cystine, tryptophan and tyrosine) are reported to be responsible for color formation with BCA. Studies with di-, tri- and tetrapeptides suggest that the extent of color formation is caused by more than the mere sum of individual color-producing functional groups. The Micro BCA Protein Assay Kit uses concentrated reagents and a protocol that utilizes an extended incubation time at an elevated temperature (60C, Test Tube Procedure only). The result is an extremely sensitive colorimetric protein assay in a test tube or microplate assay format.
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