Recombinant MT1-MMP prodomain-catalytic domain is produced as a periplasmic protein in E. coli. The pro-MT1-MMP deletion mutant consists of MT1-MMP amino acid residues Ser1. . .Gly265 followed by the His-tag sequence Leu-Val-Thr-(His)6. The calculated Mr of the recombinant proenzyme is 31 kDa. Western blot using antibodies against His-tag and MMP 14 (MT1-MMP) polyclonal antibody. Recommendation: 10 ul lysate/ lane