LEGEND MAX(TM) Human sVCAM-1/CD106 ELISA Kit with Pre-coated Plates

Human VCAM-1, also known as CD106, is an important adhesion molecule involved in leukocyte extravasation. It is a type I transmembrane glycoprotein with a molecular weight of 100 - 110 kD. It is highly conserved, with ~75% amino acid identity between mouse and human. Human VCAM-1 is expressed on a variety of cells, including neurons, endothelial cells, smooth muscle cells, fibroblasts, and macrophages. Its expression on endothelial cells can be upregulated by a variety of stimulants, such as LPS, TNF-alpha, and IL-1. VCAM-1 functions as an endothelial ligand for integrins such as VLA-4 (alpha4beta1) and LPAM-1 (alpha4beta7). VLA-4 is found on all leukocytes except neutrophils. Interaction of VCAM-1 with its receptor VLA-4 is involved in the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. It is a critical step in leukocyte extravasation. A soluble form of VCAM-1 (sVCAM-1) has been found in serum and plasma. Soluble VCAM-1 is generated due to shedding of VCAM-1 from the cell surface. Increased levels of sVCAM-1 can be detected in several disease states, including cancer, autoimmune disorders, infections, and inflammation. Therefore, sVCAM-1 can be used as a biomarker for various disease conditions. BioLegend’s LEGEND MAX(TM) Human sVCAM-1/CD106 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a capture antibody. This kit specifically quantitates human sVCAM-1 from cell culture supernatant, serum, plasma and other biological fluids. It is analytically validated with ready-to-use reagents.