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LEAF(TM) Purified anti-mouse IL-10

LEAF(TM) Purified anti-mouse IL-10

Cat no: 505011

Supplier: BioLegend
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IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-gamma, TNF-beta, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-alpha synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.
Catalogue number: 505011
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Size: 50 microg
Clone: JES5-16E3
Gene: 16153
Dilutions: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 4-8 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. * For ELISA/ELISPOT capture, it is very critical to use 0.2 M Sodium Phosphate Buffer, pH 6.5 (11.8g Na2HPO4, 16.1g NaH2PO4; q.s. to 1.0 L) as coating buffer.Note: Carbonate buffer, pH 9.5 should not be used as coating buffer for JES5-2A5. It may cause high background and lower sensitivity.
Storage buffer: The antibody solution should be stored undiluted at 4C. This LEAF(TM) solution contains no preservative; handle under aseptic conditions.
Concentration: 1 mg/ml
Antigen: E. coli-expressed, recombinant mouse IL-10
Isotype: Rat IgG2b, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Interleukin-10 (IL10), CSIF, B-TCGF, BTCGF, TGIF
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