LEAF(TM) Purified anti-mouse CD178 (FasL)

CD178 is a 40 kD member of the TNF/NGF superfamily also known as Fas ligand, FasL, Apo-1 ligand, and CD95 ligand. Cell surface CD178 is expressed on activated T cells and in testis and eye. CD178 is upregulated in activated T cells upon TCR re-engagement and has been shown to induce autocrine and paracrine T cell death. CD178 expression in the eye and testis has been shown to participate in immune privilege at these sites. CD178 can be cleaved from the surface by metalloproteases, and "soluble" CD178 may block the activities of membrane-bound CD178. CD178 binds to CD95 (Fas) to induce apoptotic cell death implicated in the maintenance of peripheral tolerance. CD178/CD95 interactions have also been implicated in the proliferation of CD8+ cells and neutrophil extravasation, chemotaxis, and survival. The MFL3 antibody recognizes CD178 in a wide array of mouse strains and has been reported to block CD178/CD95 induced apoptosis.

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SPECIFICATIONS

Catalog Number

106608

Size

500 microg

Applications

BNI, FC, IF

Hosts

Ham

Reactivities

Mouse

Clone

MFL3

Antigen

B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells

Gene Id

14103

Isotype

Armenian Hamster IgG

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ? 0.25 microg per 106 cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

1 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C. This LEAF(TM) solution contains no preservative; handle under aseptic conditions.

Alternative Names

Fas Ligand, FasL, Apo-1 Ligand, CD95 Ligand, TNFSF6

Regulatory Status

RUO