The ORF of human IZUMO1 is amplified by PCR using a high fidelity enzyme and sub-cloned into a Prokaryotic expression vector under the control of the tac promoter. The expression of the gene has been confirmed by Western blot using an anti-IZUMO1 polyclonal antibody.
Presku:
ANA10891
Size:
1ug
Gene:
284359
Target:
The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasma membrane binding and fusion.