Specific for human insulin (different antigenic determinants, Kass 6.0 x 108M-1). ?ross-reacts with human proinsulin, bovine insulin (30%) and porcine insulin. No cross-reaction with free C-peptide. Can be used for detection of insulin in two-site enzyme immunoassay. Following pairs are recommended (coating - conjugate): 7F8 - D4B8, 3A6 - 8E2.