Rockland Immunochemicals' MaxTag Histo Immunohistochemistry Kit is intended to provide a simple, reliable and convenient detection system for immunohistochemical staining. As such, the kit can be used for the identification of specific constituents in tissue sections or immobilized cells. In order to detect the reaction site, the antibody complex is labeled with an enzyme (peroxidase) that can be reacted with a suitable substrate (DAB) to give a colored product. This kit also provides for the counter staining of nuclei to reveal tissue architecture. The stain and substrate color fade slowly, if at all, allowing the slides to be stored for future use. The contents of this kit are formulated for maximum ease of use and are color coded to eliminate confusion. A sample protocol and recommended conditions for use are provided. Formaldehyde fixation is suggested as the routine initial method of choice for tissue and cell fixation. The provided protocol is a guideline. Any procedure can be altered according to specific experimental requirements. The use of both highly purified and adsorbed antibody conjugates and matched reagents reduces any lot-to-lot variation. This kit can process 100-200 immunostains on standard microscope slides and is stable for at least 1 year when stored at +4 oC.
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