This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 18 kDa in size corresponding to mature human IL-32(A) protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.