human TSLP ELISA kit (48 Wells)

Thymic Stromal Lymphopoietin (TSLP) is a 23 kDa member of the IL-7 family of a-helical cytokines (1-3). It is a monomeric glycoprotein that is synthesized as a 159 amino acid (aa) precursor (4, 5). TSLP appears to have species-specific functions. In mouse, TSLP was initially reported to act on NK cells, mast cells, and B cells, but this does not appear to occur in humans. In humans, TSLP is produced by a number of divergent cell types, all of which appear to target T cells, monocytes, and/or dendritic cells (2). On TCR-activated T cells, TSLP directly induces T cell proliferation (6). The significance of this direct action is unclear. On monocytes, TSLP is reported to induce the release of multiple chemokines that target CCR4, a receptor associated with the Th2 subset (5). TSLP is best known for its direct action on subsets of dendritic cells. In thymic medulla, HassellпїЅs corpuscle epithelium produces TSLP that acts on resident CD11c+ dendritic cells. This induces the expression of B7 family molecules on dendritic cells, which subsequently convert regional CD4+CD25+ (potentially) autoreactive T cells into CD4+CD25+FOXP3+ regulatory T cells (7). Allergen-challenged keratinocytes are also known to produce TSLP in skin where TSLP acts on Langerhans cells (CD1a+ immature dendritic cells) which then migrate to regional lymph nodes and express B7-2/CD86, CD83, high levels of MHC-II, and TARC. TARC attracts naive CD4+ T cells to the TSLP-activated Langerhans cells, and this interaction induces a Th2 phenotype. TSLP-induced Th2 cells are strong producers of IL-13, IL-5, and TNF-a, all of which promote allergic-type inflammations (8, 9).

For the quantitative determination of human Thymic Stromal Lymphopoietin (TSLP) concentrations in cell culture supernates, serum, and plasma.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TSLP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSLP present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TSLP is added to the wells and binds to the combination of capture antibody-TSLP in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of TSLP present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TSLP standard dilutions and TSLP sample concentration determined.

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