The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Lipocalin-2/NGAL present in samples reacts with the anti-Lipocalin-2/NGAL antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Lipocalin-2/NGAL antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Lipocalin-2/NGAL. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo
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