HRP Avidin

Avidin binds to biotin with high affinity. Avidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA or Western blotting. For ELISA applications, HRP will act on soluble substrates, such as ABTS or TMB, to yield a colorimetric reaction. For Western blotting or ELISPOT applications, HRP will act on precipitating substrates, such as 4CN, to yield a colorimetric reaction.

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SPECIFICATIONS

Catalog Number

405103

Size

1 ml

Applications

ELISA, ELISPOT, IHC, WB

Clone

Avidin

Dilutions

Each lot of this antibody is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:1000 - 1:5000 to determine optimal conditions. For ELISPOT, dilute at 1:500 - 1:2500 to determine optimal conditions. Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The Avidin-HRP solution should be stored undiluted at 4C. Avoid exposure to sodium azide.

Alternative Names

Avidin-Peroxidase, Avidin-Horseradish Peroxidase, Av-HRP

Regulatory Status

RUO