Principle of the assay: The Aviva human FSH ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site on a FSH molecule. An aliquot of sample containing endogenous FSH is incubated in the coated well with enzyme conjugate, which is an anti-FSH monoclonal antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of FSH in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of FSH in the sample.
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