FluoroFix Buffer

FluoroFix(TM) Buffer is aready-to-use buffer, specially formulated for fixation of immunofluorescence stained cells, optimized to stabilize tandem dyes. It can be used as the final resuspension of the cell pellet in immunofluorescence staining procedures. FluoroFix(TM) Buffer is provided as 100 mL.This quantity is sufficient for 200 tests.

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SPECIFICATIONS

Catalog Number

422101

Size

200 tests

Applications

FC, ICC

Dilutions

After final wash in the staining procedures (please refer to Cell Surface Immunofluorescence Staining Protocol), discard supernatant and resuspend the cell pellet in 0.5 ml/tube FluoroFix(TM) Buffer and analyzed with flow cytometry. If the samples can not be analyzed within 4 hours, resuspend the cell pellet in 0.5 ml/tube FluoroFix(TM) Buffer and incubate in the dark for 30 minutes at room temperature. Wash 1X with Biolegend Cell Staining Buffer (cat# 420201) and resuspend in 0.5ml/tube Cell Staining Buffer, cover the tubes. Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.

Storage Temp

Ambient RT

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The buffer solution should be stored at Room Temperature (RT).

Alternative Names

Fixative, Paraformaldehyde

Regulatory Status

RUO