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Fixation Buffer

Fixation Buffer

Cat no: 420801

Supplier: BioLegend
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Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened paraformaldehyde with low background, thus producing the greatest signal to noise ratio.
Catalogue number: 420801
Applications: Flow Cytometry, Immunocytochemistry
Size: 100 ml
Dilutions: For cell fixation, use 0.5 ml fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.
Storage buffer: This buffer solution should be stored at room temperature (RT).
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: Ambient RT
Alternative names: Fixative, Paraformaldehyde
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