FITC anti-NF-kappaB p50

NF-kappaB/p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-kappaB1) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-kappaB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-kappaB/p50 is bound (with p65) to IkappaB inhibitor in cytoplasm in inactive form. Phosphorylation of IkappaB releases and NF-kappaB which goes to the nucleus to activate gene expression. NF-kappaB can be activated with LPS, TNF-alpha, phorbol ester, or IL-1. The 4D1 monoclonal antibody reacts with human NF-kappaB p50 and has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

616706

Size

100 microg

Applications

IF

Hosts

Mouse

Reactivities

Hum

Clone

4D1

Antigen

recombinant full-length human NF-kappaB p50

Gene Id

4790

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

For immunofluorescent staining applications, a concentration range of 1-4 microg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Alternative Names

NF-kappaB p50, nuclear factor kappa light chain enhancer in B cells p50, NF-kappaB1

Regulatory Status

RUO