FITC anti-human/mouse Cutaneous Lymphocyte Antigen (CLA)

Cutaneous Lymphocyte-assiciated Antigen (CLA) is a 140 kD homodimer protein recognized by a unique mAb, HECA-452. It is expressed on T lymphocytes in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells, as well as on monocytes, granulocytes and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on majority of peripheral blood leukocytes. CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering, and rolling. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA and is suitable for staining formalin-fixed paraffin embedded tissue sections.

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SPECIFICATIONS

Catalog Number

321305

Size

25 tests

Applications

FC

Hosts

Rat

Reactivities

Hum, Mouse

Clone

HECA-452

Gene Id

949

Isotype

Rat IgM, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 microl to 5 microl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microl staining volume or per 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Alternative Names

CLA, HECA-452

Regulatory Status

RUO