FITC anti-human CD25

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor alpha chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. Soluble CD25/IL-2Ralpha is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor beta (CD122) and common gamma (CD132) chains to form a high affinity IL-2R complex.

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SPECIFICATIONS

Catalog Number

356106

Size

100 tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Clone

M-A251

Antigen

Human PHA-induced lymphocyte cells

Gene Id

3559

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 microl per million cells or 5 microl per 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Regulatory Status

RUO