Fetal human tongue tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The fetal human tongue tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the tongue tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The tongue tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.