logo
logo
ELIZEN Proinsulin ELISA Kit (96 Wells)

ELIZEN Proinsulin ELISA Kit (96 Wells)

Principle of the assay: Proinsulin kit is a sensitive two-site sandwich enzyme linked immunosorbent assay. The microtiter plates are coated with a monoclonal antibody (S2) specific for epitope at the C-peptide/insuline A chain junction. S2 is able to bind intact PI, des (31,32)-PI and split (32,33)-PI but not insulin, C-peptide and the other пїЅdesпїЅ and пїЅsplitпїЅ forms. Prior to use a blocking buffer is added to the allocated wells. An aliquot of ample is then added to the wells and incubated. The wells are then washed to remove unbound antibody and other serum compounds. In a second incubation time, an enzyme labelled anti-proinsulin antibody (S53) is incubated in the wells. This antibody is specific for the epitopes at insulin ? chain/C-peptide junction. S53 is able to bind to intact PI, des (64,65)-PI and split (65,66)-PI but not insulin, C-peptide and other пїЅdesпїЅ and пїЅsplitпїЅ forms. The combination of the two Mabs has the ability to detect only the intact human proinsulin. After washing away any unbound enzyme labelled anti-proinsulin antibody, the enzyme activity is measured by adding a chromogen substrate. The intensity of colour development is proportional to the concentration of proinsulin in the sample.

Prices direct from Aviva Systems Biology Incorporated

Quick response times

Exclusive Absave savings/discounts

SPECIFICATIONS

Size

96 Wells

Applications

ELISA

Presku

OKDA00124

Latest promotions

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

Buy any polyclonal or monoclonal antibody from our extensive range of pre-made antibodies and for a limited time only receive a $50 discount!(T&C apply:...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...