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ELISA Assay Diluent (5X)

ELISA Assay Diluent (5X)

Cat no: 421203

Supplier: BioLegend
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ELISA Assay Diluent is a 5x concentrated phosphate buffered saline solution containing (pre-screened) bovine serum. This buffer is specifically formulated and tested for ELISA applications, such as blocking antibody-coated ELISA plate microwells, diluting ELISA standards and samples, and diluting biotinylated detection antibodies or tertiary reagents (specifically Avidin-HRP or Streptavidin-HRP). The diluent contains a preservative compatible with peroxidase mediated enzyme activity.
Catalogue number: 421203
Applications: ELISA
Size: 100 ml
Dilutions: Prior to use, dilute the ELISA Assay Diluent (5X) in PBS, pH 7.4.PBS recipe: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, and 0.2 g KCl. Add deionized water to 1.0 L, filter through 0.2 microm filter, pH to 7.4
Storage buffer: This solution can be stored unopened at 4C for up to the expiration date as labeled. After opening, it is recommended to use within 30 days or to be aliquoted and stored at -20C (frost-free) or -70C for long term storage. Warm ELISA reagents to room temperature (18-25C) before use.
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: ELISA assay diluent, ELISA diluent
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