The Coomassie plus protein assay kit is a quick coomassie-binding, colorimetric method for total protein quantitation. When coomassie dye protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Performing the assay in either test tube or miscroplate format is simple?Combine a small amount of protein sample with the essay reagent, mix well, incubate briefly and measure the absorbance at 595 nm. Protein concentrations are estimated by reference to absorbances obtained for a series of standard protein dilutions, which are assayed alongside the unknown sample. When protein combined with Commassie, they will arrive at balance within 2-5 min, the complete reaction is very quick, and the combo could maintain stability for 1 hour at room temperature whose sensibility is as 4 times as Lowry method.
This kit can measure microgram of protein content and the test range of protein concentrations is 0?1500ug/
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