Chemiluminescent Western Blot Kit: DYKDDDDK (FLAG(R)) Chemiluminescent Western Blotting Kit

Use Rockland Immuno-chemicals' Anti-DYKDDDDK (FLAG(R)) Chemiluminescent Kit for Western Blotting for detection of FLAG(R) -tagged recombinant proteins by Western blot. This kit is useful for both ''Western blotting'' and ''dot blotting'' methods. After protein separation and transfer, the membrane is probed with Anti-DYKDDDDK (FLAG(R)). Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.