CFSE

CFSE is able to passively diffuse into cells. Inside the cell, its acetate groups are cleaved by intracellular esterases, and the molecules are converted to fluorescent esters. CFSE is retained within the cell and covalently couples to intracellular molecules via its succinimidyl group. Due to this covalent coupling reaction, fluorescent CFSE can be retained within the cell for an extremely long period. Also, due to this stable linkage, once the dye has been incorporated within the cell, it is not transferred to adjacent cells. CFSE is widely used for cell proliferation assays and in vivo cell tracking.

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SPECIFICATIONS

Catalog Number

422701

Size

500 microg

Applications

FC, ICC, IF

Dilutions

This lot has been tested by flow cytometric analysis of in vitro cell proliferation assay. It can be used at concentrations ranging from 0.5 - 10 microM for cell labeling. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application.

Storage Temp

-20 C

Shipping Temp

Blue Ice

Storage Buffer

This product is guaranteed for six months. Upon receipt, components should be stored desiccated at ?-20C. Reconstituted CFSE should be stored aliquoted at -20 degrees with dessicant and should be used within 6 months. Avoid repeated freeze/thaw cycles. Protect from light.

Alternative Names

5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester, CFDA SE

Regulatory Status

RUO