Brilliant Violet 650(TM) Streptavidin

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 650(TM) is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 650(TM) by 405 nm laser light induces a fluorescence maximum emission of 645 nm.

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SPECIFICATIONS

Catalog Number

405231

Size

100 microl

Applications

FC, ICC

Dilutions

Each lot of this Streptavidin-Brilliant Violet 650(TM) is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, we recommend using ?0.125 microg in 100 microl staining volume per million cells. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 650(TM) excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650(TM) is a trademark of Sirigen Group Ltd.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The streptavidin solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.

Alternative Names

SAv-Brilliant Violet 650

Regulatory Status

RUO